The aim of this study was to determine the phytochemical screening and antibacterial from leaf and callus extracts of Orthosiphon aristatus collected from different places in Tamil Nadu. Phytochemical screening involved the qualitative methods to detect the presence of terpenoids, flavonoids, phenols, tannins, steroids, quinones, saponins, cardiac glycosides and alkaloids. For the antibacterial activity different concentrations of ethanolic leaf and callus extracts were tested using the agar disc diffusion technique agaist Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The phytochemical analysis of ethanolic leaf and callus extracts of Orthosiphon aristatus was shown the availability of significant secondary metabolites such as tannins, saponins, quinones, cardiac glycosides, phenols, flavonoids, terpenoids, steroids, and alkaloids. The antibacterial activity of ethanolic leaf extract of Orthosiphon aristatus shows maximum zone of inhibition in Bacillus subtilis followed by Escherichia coli. The ethanolic callus extract shows maximum zone of inhibition in Bacillus cereus followed by Pseudomonas aeruginosa. It was concluded that the powerful antibacterial effect was attributed in the ethanolic callus extract of Orthosiphon aristatus.
2017050112.STUDIES ON PYTOCHEMICAL SCREENING ANDANTIBACTERIAL ACTIVITY FROM LEAF AND CALLUS EXTRACTS OF ORTHOSIPHON ARISTATUS (BLUME). MIQ. pdf.